Practical Flow Cytometry

Author: Howard M. Shapiro

Publisher: John Wiley & Sons

ISBN: 0471434035

Category: Science

Page: 736

View: 6692

From the reviews of the 3rd Edition... "The standard reference for anyone interested in understanding flow cytometry technology." American Journal of Clinical Oncology "...one of the most valuable of its genre and...addressed to a wide audience?written in such an attractive way, being both informative and stimulating." Trends in Cell Biology This reference explains the science and discusses the vast biomedical applications of quantitative analytical cytology using laser-activated detection and cell sorting. Now in its fourth edition, this text has been expanded to provide full coverage of the broad spectrum of applications in molecular biology and biotechnology today. New to this edition are chapters on automated analysis of array technologies, compensation, high-speed sorting, reporter molecules, and multiplex and apoptosis assays, along with fully updated and revised references and a list of suppliers.

Flow Cytometry Protocols

Author: Teresa S. Hawley

Publisher: Springer Science & Business Media

ISBN: 1592597734

Category: Science

Page: 434

View: 7083

This thoroughly revised and updated edition of a widely used practical guide to flow cytometry describes in step-by-step detail an array of time proven and cutting-edge techniques much needed in today's advanced laboratories. These readily reproducible methods deploy emerging flow cytometry technologies in many new applications, especially in the field of stem cells, functional genomics and proteomics, and microbiology. Here, the aspiring investigator will find methods for the characterization of stem/progenitor cells by monitoring the efflux of fluorescent dyes and the elucidation of signal transduction pathways using phospho-specific antibodies. There are also techniques for monitoring gene transfer and expression using fluorescent protein technology, high throughput screening for discovery of novel protein interactions, phenotypic and functional characterization of T cell subsets and precursors, and microbial flow cytometry, to highlight but some of the many useful procedures.

Flow Cytometry in Evaluation of Hematopoietic Neoplasms

A Case-Based Approach

Author: Sindhu Cherian,Brent Wood

Publisher: N.A

ISBN: 9780983706823

Category: Medical

Page: 165

View: 2286

Flow Cytometry in Evaluation of Hematopoietic Neoplasms: A Case-Based Approach is a practical, case-based guide to flow cytometric analysis in the workup of hematopoietic neoplasms presenting in the peripheral blood, marrow, lymphoid tissue, and extranodal sites. Using multi-color techniques pioneered by Brent Wood, the text demonstrates a unique approach to diagnosis of hematopoietic malignancies as well as identification of small abnormal populations in the posttherapy setting (minimal residual disease testing). The publication contains an introduction to immunophenotypic changes seen in normal hematopoiesis along with an overview of the evaluation of lymphomas, leukemias, and myeloid stem- cell neoplasms. These concepts are further illustrated by a series of 36 cases, each dedicated to a specific disease entity. Each case provides detailed, full-color images of flow cytometric dot plots that clearly outline the features of the disease, accompanied by a clinical history and thorough discussion, enabling readers to develop and work through a differential diagnosis and recognize potential diagnostic pitfalls. Take-home points for each case emphasize critical concepts in flow cytometric diagnosis. Flow Cytometry in Evaluation of Hematopoietic Neoplasms provides pathologists, residents, laboratory technologists, and hematologists with both a study guide and an atlas for regular consultation in the clinical flow cytometry laboratory. The 2008 World Health Organization (WHO) classification system, French-American-British (FAB) classifications, and 2006 Bethesda Consensus recommendations are incorporated in the text.

Clinical Immunology E-Book

Principles and Practice

Author: Robert R. Rich,Thomas A. Fleisher,William T. Shearer,Harry W. Schroeder Jr.,Anthony J. Frew,Cornelia M. Weyand

Publisher: Elsevier Health Sciences

ISBN: 0702070394

Category: Medical

Page: 1323

View: 9076

Keep abreast of the latest advances in this complex field with the 5th Edition of Clinical Immunology: Principles and Practice. This substantially revised edition by Drs. Robert R. Rich, Thomas A. Fleisher, William T. Shearer, Harry W. Schroeder, Jr., Anthony J. Frew, and Cornelia M. Weyand, offers authoritative guidance from some of the most respected global leaders in immunology, helping you navigate today’s latest knowledge and evidence-based practices that result in improved patient care. This trusted resource features sweeping content updates, rewritten chapters, a highly clinical perspective, and an easy-to-use organization designed to enhance your diagnosis and management skills in daily practice. Includes new chapters on the Microbiota in Immunity and Inflammation, Immune Responses to Fungi, and Genetics and Genomics of Immune Response. Features extensive revisions to many chapters, including the Major Histocompatibility Complex, Multiple Sclerosis, Diabetes and Related Autoimmune Diseases, Biologic Modifiers of Inflammation and Tumor Immunotherapy. Covers hot topics such as the role of genetics and genomics in immune response and immunologic disease, atherosclerosis, recurrent fever syndromes, aging and deficiencies of innate immunity, the role of microbiota in normal immune system development and the pathogenesis of immunologic and inflammatory diseases, and novel therapeutics. Addresses notable advances in key areas such as the importance of the microbiota to normal immune system development and to the pathogenesis of immunologic and inflammatory diseases; relationships between the innate and adaptive immune systems; progress in rapid and cost-effective genomics; cell signaling pathways and the structure of cell-surface molecules; and many more. Summarizes promising research and development anticipated over the next 5-10 years with "On the Horizon" boxes and discussion of translational research.

Flow Cytometry

First Principles

Author: Alice Longobardi Givan

Publisher: John Wiley & Sons

ISBN: 1118688392

Category: Science

Page: 296

View: 4591

Flow cytometry continually amazes scientists with its ever-expanding utility. Advances in flow cytometry have opened new directions in theoretical science, clinical diagnosis, and medical practice. The new edition of Flow Cytometry: First Principles provides a thorough update of this now classic text, reflecting innovations in the field while outlining the fundamental elements of instrumentation, sample preparation, and data analysis. Flow Cytometry: First Principles, Second Edition explains the basic principles of flow cytometry, surveying its primary scientific and clinical applications and highlighting state-of-the-art techniques at the frontiers of research. This edition contains extensive revisions of all chapters, including new discussions on fluorochrome and laser options for multicolor analysis, an additionalsection on apoptosis in the chapter on DNA, and new chapters onintracellular protein staining and cell sorting, including high-speed sorting and alternative sorting methods, as well as traditional technology. This essential resource: Assumes no prior knowledge of flow cytometry Progresses with an informal, engaging lecture style from simpleto more complex concepts Offers a clear introduction to new vocabulary, principles of instrumentation, and strategies for data analysis Emphasizes the theory relevant to all flow cytometry, with examples from a variety of clinical and scientific fields Flow Cytometry: First Principles, Second Edition provides scientists, clinicians, technologists, and students with the knowledge necessary for beginning the practice of flow cytometry and for understanding related literature.

Flow Cytometry of Hematological Malignancies

Author: Claudio Ortolani

Publisher: John Wiley & Sons

ISBN: 144433588X

Category: Medical

Page: 311

View: 977

Flow Cytometry of Hematological Malignancies contains an array of graphical outputs produced by the technique in the study of the most (and the least) common diseases. The images included allow you to compare your own results with a third party reference pattern. There is a detailed description of the main leukocyte antigens, together with a description of their distribution amongst normal and abnormal blood cells. The book also provides a comprehensive description of the phenotype of every neoplastic blood disease recorded in the WHO classification system, including all the instructions needed to recognise and classify even the least common entity. Designed to be practical, the book is perfect for quick consultation and is divided into two main sections. Section I deals with the direct object of immunophenotyping, and Section II deals with the ultimate target of the analysis. More than 50 antigens are covered and every antigen is dealt with in three main parts: general features, cytometric features and practical hints. This authoritative and state–of–the–art reference will be invaluable for clinicians directly involved in the diagnosis and analysis of hematological diseases, including hematologists, hematopathologists, oncologists, pathologists and technicians working in diagnostic laboratories.

Clinical Virology Manual

Author: Steven C. Specter,Richard L. Hodinka,Danny L. Wiedbrauk,Stephen A. Young

Publisher: American Society for Microbiology Press

ISBN: 155581462X

Category: Medical

Page: 725

View: 1248

A comprehensive and updated volume for the clinical virologist. • Details laboratory procedures for detecting and handling viruses, from specimen requirements and quality assurance to virus detection and identification, from the fundamentals through the latest molecular methods. • Presents the most current knowledge on the wide range of specific viral pathogens. • Includes information on services provided by federal and state public health virology laboratories. • Provides essential information for clinicians and laboratory virologists.

Molecular Biology of the Cell

Author: Bruce Alberts

Publisher: Garland Science

ISBN: 1317563743

Category: Science

Page: 1464

View: 7968

As the amount of information in biology expands dramatically, it becomes increasingly important for textbooks to distill the vast amount of scientific knowledge into concise principles and enduring concepts.As with previous editions, Molecular Biology of the Cell, Sixth Edition accomplishes this goal with clear writing and beautiful illustrations. The Sixth Edition has been extensively revised and updated with the latest research in the field of cell biology, and it provides an exceptional framework for teaching and learning. The entire illustration program has been greatly enhanced.Protein structures better illustrate structure–function relationships, icons are simpler and more consistent within and between chapters, and micrographs have been refreshed and updated with newer, clearer, or better images. As a new feature, each chapter now contains intriguing openended questions highlighting “What We Don’t Know,” introducing students to challenging areas of future research. Updated end-of-chapter problems reflect new research discussed in the text, and these problems have been expanded to all chapters by adding questions on developmental biology, tissues and stem cells, pathogens, and the immune system.

Flow Cytometry

Principles and Applications

Author: Marion G. Macey

Publisher: Springer Science & Business Media

ISBN: 1597454516

Category: Medical

Page: 294

View: 3025

Flow cytometry forms an integral part of both basic biological research and clinical diagnosis in pathology. This straightforward new volume provides a clear, easy-to-read, and practical manual for both clinicians and non-clinicians at all levels of their careers. The chapter topics range from basic principles to more advanced subjects, such as apoptosis and cell sorting. The book charts the history, development and basic principles of flow cytometry.

Cytology

Diagnostic Principles and Clinical Correlates

Author: Edmund S. Cibas,Barbara S. Ducatman

Publisher: Elsevier Health Sciences

ISBN: 1416053298

Category: Medical

Page: 537

View: 2355

This new edition examines the latest diagnostic techniques for the interpretation of a complete range of cytological specimens. It is concise, yet covers all of the organ systems in which the procedure is used, with the number of pages devoted to each body site proportional to the clinical relevance of cytology for that site. Inside, you'll find new information on ductal lavage cytology and expanded coverage of FNA performance, keeping you current with the newest procedures. Over 700 full-color illustrations provide you with a real-life perspective of a full range of cytologic findings. Each chapter includes a discussion of indications and methods, along with a section on differential diagnosis accompanied by ancillary diagnostic techniques such as immunohistochemistry and molecular biology, where appropriate. As an Expert Consult title, it includes online access to the complete text of the book, fully searchable-as well as all of the images downloadable for your personal use-at www.expertconsult.com. "We like this book and use it frequently, thank you Dr Cibas" Reviewed by: PathLab.org Date: June 2014 Offers comprehensive coverage of everyday diagnostic work in a concise format for a practical benchside manual. Covers every type of cytology-gynecology, non-gynecology, and FNA. Presents an in-depth differential diagnosis discussion for all major entities. Examines the role of special techniques such as immunohistochemistry, flow cytometry, and molecular biology in resolving difficulties in interpretation and diagnosis. Provides an in-depth analysis of common diagnostic pitfalls to assist with daily signing out and reporting. Features coverage of patient management in discussions of pertinent clinical features. Uses capsule summaries featuring easy-to-read bulleted text that provide a quick review of key differential diagnoses, diagnostic pitfalls, cytomorphologic features, and tissue acquisition protocols for specific entities. Includes over 700 full-color illustrations that provide you with a real-life perspective of a full range of cytologic findings. Features anytime, anywhere online access to the complete text of the book, fully searchable-as well as all of the images downloadable for your personal use-at www.expertconsult.com. Covers automated cytology and HPV testing in Cervical and Vaginal Cytology chapter, providing an up-to-date reference on the techniques used in today's labs. Offers new information on ductal lavage cytology and expanded coverage of FNA performance, keeping you current with the newest procedures. Discusses the implementation of proficiency testing and changes in laboratory inspection and accreditation. Includes recommendations from the 2008 National Cancer Institute Thyroid Fine Needle Aspiration State of the Science Conference. Your purchase entitles you to access the web site until the next edition is published, or until the current edition is no longer offered for sale by Elsevier, whichever occurs first. If the next edition is published less than one year after your purchase, you will be entitled to online access for one year from your date of purchase. Elsevier reserves the right to offer a suitable replacement product (such as a downloadable or CD-ROM-based electronic version) should access to the web site be discontinued.

Flow Cytometry Protocols

Author: Teresa S. Hawley,Robert Hawley

Publisher: Humana Press

ISBN: 9781493956623

Category: Science

Page: 485

View: 5871

With rapid improvements in instrumentation, lasers, fluorophores, and data analysis software, flow cytometry is riding the crest of unprecedented, innovative advances. This thoroughly revised and up-to-date third edition of Flow Cytometry Protocols highlights the expanding contribution of flow cytometry to basic biological research and diagnostic medicine. Written by leading experts in the field, the book presents cutting-edge topics such as polychromatic, quantitative, and high throughput flow cytometry, novel multiparametric data analysis which breaks the dimensionality barrier, standard practice and safety measures for aerosol-generating cell sorting, conventional and imaging flow cytometry as well as minimalist imaging cytometry. As a volume in the highly successful Methods in Molecular BiologyTM series, chapters contain brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and extensive notes on troubleshooting and avoiding known pitfalls. Authoritative and comprehensive, Flow Cytometry Protocols, Third Edition presents established as well as new flow cytometric methodologies in order to introduce beginning users to basic applications while opening new avenues of innovation for seasoned users.

High Throughput Screening for Food Safety Assessment

Biosensor Technologies, Hyperspectral Imaging and Practical Applications

Author: Arun K. Bhunia,Moon S. Kim,Chris R. Taitt

Publisher: Elsevier

ISBN: 0857098071

Category: Technology & Engineering

Page: 550

View: 2667

Recent advances in array-based detectors and imaging technologies have provided high throughput systems that can operate within a substantially reduced timeframe and other techniques that can detect multiple contaminants at one time. These technologies are revolutionary in terms of food safety assessment in manufacturing, and will also have a significant impact on areas such as public health and food defence. This book summarizes the latest research and applications of sensor technologies for online and high throughput screening of food. The book first introduces high throughput screening strategies and technology platforms, and discusses key issues in sample collection and preparation. The subsequent chapters are then grouped into four sections: Part I reviews biorecognition techniques; Part II covers the use of optical biosensors and hyperspectral imaging in food safety assessment; Part III focuses on electrochemical and mass-based transducers; and finally Part IV deals with the application of these safety assessment technologies in specific food products, including meat and poultry, seafood, fruits and vegetables. Summarises the latest research on sensor technologies for online and high-throughput screening of food Covers high-throughput screening and the current and forecast state of rapid contaminant detection technologies Looks at the use of optical and electrochemical biosensors and hyperspectral imaging in food safety assessment and the application of these technologies in specific food products

Single Cell Analysis

Contemporary Research and Clinical Applications

Author: J. Paul Robinson,Andrea Cossarizza

Publisher: Springer

ISBN: 9811044996

Category: Technology & Engineering

Page: 266

View: 4931

This book highlights the current state of the art in single cell analysis, an area that involves many fields of science – from clinical hematology, functional analysis and drug screening, to platelet and microparticle analysis, marine biology and fundamental cancer research. This book brings together an eclectic group of current applications, all of which have a significant impact on our current state of knowledge. The authors of these chapters are all pioneering researchers in the field of single cell analysis. The book will not only appeal to those readers more focused on clinical applications, but also those interested in highly technical aspects of the technologies. All of the technologies identified utilize unique applications of photon detection systems.

Handbook of Biological Confocal Microscopy

Author: James Pawley

Publisher: Springer Science & Business Media

ISBN: 1461571332

Category: Medical

Page: 232

View: 3898

In 1987 the Electron Microscopy Society of America (EMSA) going to drive important scientific discoveries across wide areas under the leadership of J. P. Revel (Cal Tech) initiated a major of physiology, cellular biology and neurobiology. They had been program to present a discussion of recent advances in light looking for a forum in which they could advance the state of microscopy as part of the annual meeting. The result was three the art of confocal microscopy, alert manufacturers to the lim special LM sessions at the Milwaukee meeting in August 1988: itations of current instruments, and catalyze progress toward The LM Forum, organized by me, and Symposia on Confocal new directions in confocal instrument development. LM, organized by G. Schatten (Madison), and on Integrated These goals were so close to those of the EMSA project that Acoustic/LM/EM organized by C. Rieder (Albany). In addition, the two groups decided to join forces with EMSA to provide there was an optical micro-analysis session emphasizing Raman the organization and the venue for a Confocal Workshop and techniques, organized by the Microbeam Analysis Society, for NSF to provide the financial support for the speakers expenses a total of 40 invited and 30 contributed papers on optical tech and for the publication of extended abstracts.

Ioachim's Lymph Node Pathology

Author: Harry L. Ioachim,L. Jeffrey Medeiros

Publisher: Lippincott Williams & Wilkins

ISBN: 9780781775960

Category: Medical

Page: 680

View: 2372

Now in its Fourth Edition, this comprehensive, practical, and thoroughly illustrated reference offers valuable guidance in the diagnostic interpretation of lymph node biopsies. It provides encyclopedic coverage of all the various nonmalignant lesions, lymphomas, other neoplasms, and metastatic tumors in lymph nodes. The discussion of each pathologic entity includes definition, clinical syndrome, histopathology, and differential diagnosis. This edition has more than 700 illustrations, including over 600 in full color. Dr. Ioachim is joined by a new co-editor, L. Jeffrey Medeiros, MD, from the University of Texas MD Anderson Cancer Center. All chapters have been extensively revised and a new chapter on genetics has been added. A companion Website will offer the fully searchable online text and an image bank.

Mastering Patient Flow

More Idead to Increase Efficiency and Earnings

Author: Elizabeth W. Woodcock

Publisher: Medical Group Management Assn

ISBN: 9781568292281

Category: Medical

Page: 292

View: 6341

Tackle the issues of space capacity, utilization, patient flow and technology in this best-selling book for medical practice management.

Comprehensive Biotechnology

Author: N.A

Publisher: Newnes

ISBN: 0080885047

Category: Science

Page: 5320

View: 5642

The second edition of Comprehensive Biotechnology continues the tradition of the first inclusive work on this dynamic field with up-to-date and essential entries on the principles and practice of biotechnology. The integration of the latest relevant science and industry practice with fundamental biotechnology concepts is presented with entries from internationally recognized world leaders in their given fields. With two volumes covering basic fundamentals, and four volumes of applications, from environmental biotechnology and safety to medical biotechnology and healthcare, this work serves the needs of newcomers as well as established experts combining the latest relevant science and industry practice in a manageable format. It is a multi-authored work, written by experts and vetted by a prestigious advisory board and group of volume editors who are biotechnology innovators and educators with international influence. All six volumes are published at the same time, not as a series; this is not a conventional encyclopedia but a symbiotic integration of brief articles on established topics and longer chapters on new emerging areas. Hyperlinks provide sources of extensive additional related information; material authored and edited by world-renown experts in all aspects of the broad multidisciplinary field of biotechnology Scope and nature of the work are vetted by a prestigious International Advisory Board including three Nobel laureates Each article carries a glossary and a professional summary of the authors indicating their appropriate credentials An extensive index for the entire publication gives a complete list of the many topics treated in the increasingly expanding field

Clinical Informatics Board Review and Self Assessment

Author: Scott Mankowitz

Publisher: Springer

ISBN: 3319637665

Category: Medical

Page: 343

View: 2624

The book offers an introduction to all the informatics concepts that are represented on the Clinical Informatics Board Examination The core and direction of this book is to mirror the model of clinical informatics which is used by the American Board of Preventive Medicine to create their exam. Unlike any other text on the market, the book includes simulated exam questions, to help the reader asses his knowledge and focus his study. Clinical Informatics Board Review and Self Assessment is a thorough practical assistant to refine the reader's knowledge regarding this youngest and possibly broadest fields of medicine.

Flow Cytometry in Clinical Diagnosis

Author: David E. Keren

Publisher: American Society of Clinical Oncology

ISBN: 9780891895480

Category: Flow cytometry

Page: 384

View: 8421

The new edition promises a completely current overview of this increasingly commonplace technology. With every chapter either completely reworked or significantly updated to include the latest developments and new contributions from 10 new international authorities, this will be the go-to resource on clinical flow cytometry for years to come. More than that it promises to deliver a uniquely engaging learning experience with a bonus CD filled with real-world cases and interpretations. Drs. Carey and McCoy bring the new edition of 'Flow Cytometry in Clinical Diagnosis' alive with the bonus CD, which includes data from actual flow analysis and patient samples for 39 cases.