Calculations for Molecular Biology and Biotechnology

Author: Frank H. Stephenson

Publisher: Academic Press

ISBN: 0128025980

Category: Computers

Page: 496

View: 8005

Calculations in Molecular Biology and Biotechnology, Third Edition, helps researchers utilizing molecular biology and biotechnology techniques—from student to professional—understand which type of calculation to use and why. Research in biotechnology and molecular biology requires a vast amount of calculations. Results of one data set become the basis of the next. An error of choosing the wrong type of equation can turn what would have been a successful research project or weeks of labor and research into a veritable house of cards. It could be how you calculated the medium in which you test your sample to calculating how long it takes a sample to grow to calculating the synthesis of multiple variables. In one easy to use reference, Stephenson reviews the mathematics and statistics related to the day-to-day functions of biotechnology and molecular biology labs, which is a sticking point for many students, technicians, and researchers. The book covers all of the basic mathematical and statistical needs for students and professionals, providing them with a useful tool for their work. Features comprehensive calculations in biotechnology and molecular biology experiments from start to finish Provides coverage ranging from basic scientific notations to complex subjects like nucleic acid chemistry and recombinant DNA technology Includes recent applications of the procedures and computations in clinical, academic, industrial, and basic research laboratories cited throughout the text Features new coverage of digital PCR and protein quantification including chromatography and radiolabelling of proteins Includes more sample problems in every chapter for readers to practice concepts

Calculations for Molecular Biology and Biotechnology

A Guide to Mathematics in the Laboratory

Author: Frank H. Stephenson

Publisher: Academic Press

ISBN: 9780123756916

Category: Science

Page: 460

View: 6318

Calculations for Molecular Biology and Biotechnology: A Guide to Mathematics in the Laboratory, Second Edition, provides an introduction to the myriad of laboratory calculations used in molecular biology and biotechnology. The book begins by discussing the use of scientific notation and metric prefixes, which require the use of exponents and an understanding of significant digits. It explains the mathematics involved in making solutions; the characteristics of cell growth; the multiplicity of infection; and the quantification of nucleic acids. It includes chapters that deal with the mathematics involved in the use of radioisotopes in nucleic acid research; the synthesis of oligonucleotides; the polymerase chain reaction (PCR) method; and the development of recombinant DNA technology. Protein quantification and the assessment of protein activity are also discussed, along with the centrifugation method and applications of PCR in forensics and paternity testing. Topics range from basic scientific notations to complex subjects like nucleic acid chemistry and recombinant DNA technology Each chapter includes a brief explanation of the concept and covers necessary definitions, theory and rationale for each type of calculation Recent applications of the procedures and computations in clinical, academic, industrial and basic research laboratories are cited throughout the text New to this Edition: Updated and increased coverage of real time PCR and the mathematics used to measure gene expression More sample problems in every chapter for readers to practice concepts

Calculations for Molecular Biology and Biotechnology

A Guide to Mathematics in the Laboratory

Author: Frank H. Stephenson

Publisher: Elsevier

ISBN: 0080500617

Category: Science

Page: 302

View: 5724

Calculations in Molecular Biology and Biotechnology: A Guide to Mathematics in the Laboratory is the first comprehensive guide devoted exclusively to calculations encountered in the genetic engineering laboratory. Mathematics, as a vital component of the successful design and interpretation of basic research, is used daily in laboratory work. This guide, written for students, technicians, and scientists, provides example calculations for the most frequently confronted problems encountered in gene discovery and analysis. The text and sample calculations are written in an easy-to-follow format. It is the perfect laboratory companion for anyone working in DNA manipulation and analysis. *A comprehensive guide to calculations for a wide variety of problems encountered in the basic research laboratory. * Example calculations are worked through from start to finish in easy-to-follow steps * Key chapters devoted to calculations encountered when working with bacteria, phage, PCR, radioisotopes, recombinant DNA, centrifugation, oligonucleotides, protein, and forensic science. *Written for students and laboratory technicians but a useful reference for the more experienced researcher. *A valuable teaching resource.

Principles and Techniques of Biochemistry and Molecular Biology

Author: Keith Wilson,John Walker

Publisher: Cambridge University Press

ISBN: 1107393493

Category: Science

Page: N.A

View: 7690

This best-selling undergraduate textbook provides an introduction to key experimental techniques from across the biosciences. It uniquely integrates the theories and practices that drive the fields of biology and medicine, comprehensively covering both the methods students will encounter in lab classes and those that underpin recent advances and discoveries. Its problem-solving approach continues with worked examples that set a challenge and then show students how the challenge is met. New to this edition are case studies, for example, that illustrate the relevance of the principles and techniques to the diagnosis and treatment of individual patients. Coverage is expanded to include a section on stem cells, chapters on immunochemical techniques and spectroscopy techniques, and additional chapters on drug discovery and development, and clinical biochemistry. Experimental design and the statistical analysis of data are emphasised throughout to ensure students are equipped to successfully plan their own experiments and examine the results obtained.

Biochemical Engineering and Biotechnology

Author: Ghasem Najafpour

Publisher: Elsevier

ISBN: 0444633774

Category: Technology & Engineering

Page: 668

View: 4759

Biochemical Engineering and Biotechnology, 2nd Edition, outlines the principles of biochemical processes and explains their use in the manufacturing of every day products. The author uses a diirect approach that should be very useful for students in following the concepts and practical applications. This book is unique in having many solved problems, case studies, examples and demonstrations of detailed experiments, with simple design equations and required calculations. Covers major concepts of biochemical engineering and biotechnology, including applications in bioprocesses, fermentation technologies, enzymatic processes, and membrane separations, amongst others Accessible to chemical engineering students who need to both learn, and apply, biological knowledge in engineering principals Includes solved problems, examples, and demonstrations of detailed experiments with simple design equations and all required calculations Offers many graphs that present actual experimental data, figures, and tables, along with explanations

Lab Math

A Handbook of Measurements, Calculations, and Other Quantitative Skills for Use at the Bench

Author: Dany Spencer Adams

Publisher: CSHL Press

ISBN: 9780879696344

Category: Mathematics

Page: 275

View: 2066

Work at the biology bench requires an ever-increasing knowledge of mathematical methods and formulae. In Lab Math,Dany Spencer Adams has compiled the most common mathematical concepts and methods in molecular biology, and provided clear, straightforward guidance on their application to research investigations. Subjects range from basics such as scientific notation and measuring and making solutions, to more complex activities like quantifying and designing nucleic acids and analyzing protein activity. Tips on how to present mathematical data and statistical analysis are included. A reference section features useful tables, conversion charts and “plug and chug” equations for experimental procedures. This volume is an excellent, structured source of information that in many laboratories is often scattered and informally organized.

Analytical Techniques in Biochemistry and Molecular Biology

Author: Rajan Katoch

Publisher: Springer Science & Business Media

ISBN: 9781441997852

Category: Science

Page: 441

View: 3667

Advances in biochemistry now allow us to control living systems in ways that were undreamt of a decade ago. This volume guides researchers and students through the full spectrum of experimental protocols used in biochemistry, plant biology and biotechnology.

A Hands-On Introduction to Forensic Science

Cracking the Case

Author: Mark Okuda,Frank H. Stephenson, PhD.

Publisher: CRC Press

ISBN: 1482234904

Category: Law

Page: 508

View: 8826

One failing of many forensic science textbooks is the isolation of chapters into compartmentalized units. This format prevents students from understanding the connection between material learned in previous chapters with that of the current chapter. Using a unique format, A Hands-On Introduction to Forensic Science: Cracking the Case approaches the topic of forensic science from a real-life perspective in a way that these vital connections are encouraged and established. The book utilizes an ongoing fictional narrative throughout, entertaining students as it provides hands-on learning in order to "crack the case." As two investigators try to solve a missing persons case, each succeeding chapter reveals new characters, new information, and new physical evidence to be processed. A full range of topics are covered, including processing the crime scene, lifting prints, trace and blood evidence, DNA and mtDNA sequencing, ballistics, skeletal remains, and court testimony. Following the storyline, students are introduced to the appropriate science necessary to process the physical evidence, including math, physics, chemistry, and biology. The final element of each chapter includes a series of cost-effective, field-tested lab activities that train students in processing, analyzing, and documenting the physical evidence revealed in the narrative. Practical and realistic in its approach, this book enables students to understand how forensic science operates in the real world.

Basic Laboratory Calculations for Biotechnology

Author: Lisa A. Seidman

Publisher: Benjamin-Cummings Publishing Company

ISBN: N.A

Category: Mathematics

Page: 482

View: 2971

To succeed in the lab, it is crucial to be comfortable with the math calculations that are part of everyday work. This accessible introduction to common laboratory techniques focuses on the basics, helping even readers with good math skills to practice the most frequently encountered types of problems. Discusses very common laboratory problems, all applied to real situations. Explores multiple strategies for solving problems for a better understanding of the underlying math. Includes hundreds of practice problems, all with solutions and many with boxed, complete explanations; plus hundreds of “story problems” relating to real situations in the lab. MARKET: A useful review for biotechnology laboratory professionals.

Calculations for Molecular Biology and Biotechnology, A Guide to Mathematics in the Laboratory 2e

Biology, Molecular biology

Author: CTI Reviews

Publisher: Cram101 Textbook Reviews

ISBN: 1467203106

Category: Education

Page: 17

View: 9913

Facts101 is your complete guide to Calculations for Molecular Biology and Biotechnology, A Guide to Mathematics in the Laboratory 2e. In this book, you will learn topics such as Cell Growth, Working with Bacteriophages, Nucleic Acid Quantification, and Labeling Nucleic Acids with Radioisotopes plus much more. With key features such as key terms, people and places, Facts101 gives you all the information you need to prepare for your next exam. Our practice tests are specific to the textbook and we have designed tools to make the most of your limited study time.

DNA

How the Biotech Revolution Is Changing the Way We Fight Disease

Author: Frank H. Stephenson

Publisher: Prometheus Books

ISBN: 1615924280

Category: Medical

Page: N.A

View: 8016

In the fall of 2005, leading scientists from the National Cancer Institute announced the beginning of the cancer genome atlas project, a large-scale endeavor to map every gene implicated in cancer and the first step toward development of new therapies for treating this still baffling disease. This spin-off of the human genome project is only the latest exciting research advance in a decades-long quest to fully understand the biochemistry of the human body and thereby gain insights into the secrets of health, disease, and aging. Biochemist and veteran lab researcher Frank H. Stephenson tells the compelling story of how scientists on many fronts are succeeding in the battle against disease. With a gift for making the complexities of genetics and biochemistry understandable to the average reader, Stephenson offers a fascinating tour of the mechanisms of our body and the therapeutic techniques that are gaining in sophistication and effectiveness every year. From heart disease to AIDS and cancer, he helps you understand how the tools of biotechnology are being used to combat our most common afflictions. Stephenson examines a wide variety of health threats and illnesses: HIV infection, the many forms of cancer, asthma, diabetes, Alzheimer's, obesity, and even erectile dysfunction. Each is discussed in terms of its root cause and treatment in plain, jargon-free language that not only educates but also entertains. This is the ideal primer on the biotechnology revolution for the layperson. Stephenson offers many insights into both the diseases that destroy health and the great promises that biotechnology offers for preserving and prolonging a healthy life.Frank H. Stephenson, Ph.D. (Pacifica, CA), has worked in the biotechnology industry for many years. He is the author of Calculations for Molecular Biology and Biotechnology, as well as book chapters and articles on biochemistry.

Recombinant DNA and Biotechnology

A Guide for Students

Author: Helen Kreuzer,Adrianne Massey

Publisher: Wiley-Blackwell

ISBN: 9781555811761

Category: Science

Page: 431

View: 3880

Written in clear, easy–to–understand language, this best–selling reference text and activities manual offers easy–to–implement lessons and classroom activities. Part I covers basic molecular biology, and Part II offers imaginative dry labs and wet labs that can be done by both college and precollege students. Part III is an innovative section addressing the social issues and public concerns of biotechnology. Extensive appendixes provide important background information on basic laboratory techniques and teaching resources, including overhead masters and templates. Adopted by numerous school systems, this unique book is an outgrowth of molecular biology and biotechnology teaching workshops. All of the exercises and lab activities have been extensively tested in the classroom by hundreds of high school teachers. Recombinant DNA and Biotechnology is designed to interest an international teaching audience and will enable all instructors to teach a reasonable amount of molecular biology and genetic engineering to students. No other book makes it so easy or compelling for teachers to incorporate the "new biology" into their biology, biological sciences, or general science curriculum. Recombinant DNA and Biotechnology: A Guide for Teachers will enable college and precollege teachers to plan and conduct an exciting and contemporary course on the basic principles, essential laboratory activities, and relevant social issues and concerns attendant to today′s molecular biology revolution. In addition to the complete text of the student edition, A Guide for Teachers also contains the answers to all discussion questions and extra background information and material on the scientific principles involved.

Comprehensive Biotechnology

Author: N.A

Publisher: Newnes

ISBN: 0080885047

Category: Science

Page: 5320

View: 4194

The second edition of Comprehensive Biotechnology continues the tradition of the first inclusive work on this dynamic field with up-to-date and essential entries on the principles and practice of biotechnology. The integration of the latest relevant science and industry practice with fundamental biotechnology concepts is presented with entries from internationally recognized world leaders in their given fields. With two volumes covering basic fundamentals, and four volumes of applications, from environmental biotechnology and safety to medical biotechnology and healthcare, this work serves the needs of newcomers as well as established experts combining the latest relevant science and industry practice in a manageable format. It is a multi-authored work, written by experts and vetted by a prestigious advisory board and group of volume editors who are biotechnology innovators and educators with international influence. All six volumes are published at the same time, not as a series; this is not a conventional encyclopedia but a symbiotic integration of brief articles on established topics and longer chapters on new emerging areas. Hyperlinks provide sources of extensive additional related information; material authored and edited by world-renown experts in all aspects of the broad multidisciplinary field of biotechnology Scope and nature of the work are vetted by a prestigious International Advisory Board including three Nobel laureates Each article carries a glossary and a professional summary of the authors indicating their appropriate credentials An extensive index for the entire publication gives a complete list of the many topics treated in the increasingly expanding field

Introduction to Computational Biology

Maps, Sequences and Genomes

Author: Michael S. Waterman

Publisher: CRC Press

ISBN: 1351437089

Category: Mathematics

Page: 448

View: 6081

Biology is in the midst of a era yielding many significant discoveries and promising many more. Unique to this era is the exponential growth in the size of information-packed databases. Inspired by a pressing need to analyze that data, Introduction to Computational Biology explores a new area of expertise that emerged from this fertile field- the combination of biological and information sciences. This introduction describes the mathematical structure of biological data, especially from sequences and chromosomes. After a brief survey of molecular biology, it studies restriction maps of DNA, rough landmark maps of the underlying sequences, and clones and clone maps. It examines problems associated with reading DNA sequences and comparing sequences to finding common patterns. The author then considers that statistics of pattern counts in sequences, RNA secondary structure, and the inference of evolutionary history of related sequences. Introduction to Computational Biology exposes the reader to the fascinating structure of biological data and explains how to treat related combinatorial and statistical problems. Written to describe mathematical formulation and development, this book helps set the stage for even more, truly interdisciplinary work in biology.

Biotechnology for Beginners

Author: Reinhard Renneberg,Viola Berkling,Vanya Loroch

Publisher: Academic Press

ISBN: 0128012730

Category: Science

Page: 464

View: 7725

Biotechnology for Beginners, Second Edition, presents the latest information and developments from the field of biotechnology—the applied science of using living organisms and their by-products for commercial development—which has grown and evolved to such an extent over the past few years that increasing numbers of professionals work in areas that are directly impacted by the science. For the first time, this book offers an exciting and colorful overview of biotechnology for professionals and students in a wide array of the life sciences, including genetics, immunology, biochemistry, agronomy, and animal science. This book also appeals to the lay reader without a scientific background who is interested in an entertaining and informative introduction to the key aspects of biotechnology. Authors Renneberg and Demain discuss the opportunities and risks of individual technologies and provide historical data in easy-to-reference boxes, highlighting key topics. The book covers all major aspects of the field, from food biotechnology to enzymes, genetic engineering, viruses, antibodies, and vaccines, to environmental biotechnology, transgenic animals, analytical biotechnology, and the human genome. This stimulating book is the most user-friendly source for a comprehensive overview of this complex field. Provides accessible content to the lay reader who does not have an extensive scientific background Includes all facets of biotechnology applications Covers articles from the most respected scientists, including Alan Guttmacher, Carl Djerassi, Frances S. Ligler, Jared Diamond, Susan Greenfield, and more Contains a summary, annotated references, links to useful web sites, and appealing review questions at the end of each chapter Presents more than 600 color figures and over 100 illustrations Written in an enthusiastic and engaging style unlike other existing theoretical and dry-style biotechnology books

Molecular Biology of the Cell

Author: Bruce Alberts

Publisher: Garland Science

ISBN: 1317563743

Category: Science

Page: 1464

View: 8891

As the amount of information in biology expands dramatically, it becomes increasingly important for textbooks to distill the vast amount of scientific knowledge into concise principles and enduring concepts.As with previous editions, Molecular Biology of the Cell, Sixth Edition accomplishes this goal with clear writing and beautiful illustrations. The Sixth Edition has been extensively revised and updated with the latest research in the field of cell biology, and it provides an exceptional framework for teaching and learning. The entire illustration program has been greatly enhanced.Protein structures better illustrate structure–function relationships, icons are simpler and more consistent within and between chapters, and micrographs have been refreshed and updated with newer, clearer, or better images. As a new feature, each chapter now contains intriguing openended questions highlighting “What We Don’t Know,” introducing students to challenging areas of future research. Updated end-of-chapter problems reflect new research discussed in the text, and these problems have been expanded to all chapters by adding questions on developmental biology, tissues and stem cells, pathogens, and the immune system.

Protein Bioinformatics

From Sequence to Function

Author: M. Michael Gromiha

Publisher: Academic Press

ISBN: 0123884241

Category: Computers

Page: 339

View: 5514

One of the most pressing tasks in biotechnology today is to unlock the function of each of the thousands of new genes identified every day. Scientists do this by analyzing and interpreting proteins, which are considered the task force of a gene. This single source reference covers all aspects of proteins, explaining fundamentals, synthesizing the latest literature, and demonstrating the most important bioinformatics tools available today for protein analysis, interpretation and prediction. Students and researchers of biotechnology, bioinformatics, proteomics, protein engineering, biophysics, computational biology, molecular modeling, and drug design will find this a ready reference for staying current and productive in this fast evolving interdisciplinary field. Explains all aspects of proteins including sequence and structure analysis, prediction of protein structures, protein folding, protein stability, and protein interactions Presents a cohesive and accessible overview of the field, using illustrations to explain key concepts and detailed exercises for students.

Environmental Biotechnology

A Biosystems Approach

Author: Daniel Vallero

Publisher: Academic Press

ISBN: 0124078974

Category: Science

Page: 746

View: 7397

Environmental Biotechnology: A Biosystems Approach, Second Edition presents valuable information on how biotechnology has acted as a vital buffer among people, pollution, and the environment. It answers the most important questions on the topic, including how, and why, a knowledge and understanding of the physical, chemical, and biological principles of the environment must be achieved in order to develop biotechnology applications. Most texts address either the applications or the implications of biotechnology. This book addresses both. The applications include biological treatment and other environmental engineering processes. The risks posed by biotechnologies are evaluated from both evidence-based and precautionary perspectives. Using a systems biology approach, the book provides a context for researchers and practitioners in environmental science that complements guidebooks on the necessary specifications and criteria for a wide range of environmental designs and applications. Users will find crucial information on the topics scientific researchers must evaluate in order to develop further technologies. Provides a systems approach to biotechnologies which includes the physical, biological, and chemical processes in context Presents relevant case studies on cutting-edge technologies, such as nanobiotechnologies and green engineering Addresses both the applications and implications of biotechnologies by following the lifecycle of a variety of established and developing biotechnologies Includes crucial information on the topics scientific researchers must evaluate in order to develop further technologies

Wilson and Walker's Principles and Techniques of Biochemistry and Molecular Biology

Author: Andreas Hofmann,Samuel Clokie

Publisher: Cambridge University Press

ISBN: 1108365256

Category: Science

Page: N.A

View: 7172

Bringing this best-selling textbook right up to date, the new edition uniquely integrates the theories and methods that drive the fields of biology, biotechnology and medicine, comprehensively covering both the techniques students will encounter in lab classes and those that underpin current key advances and discoveries. The contents have been updated to include both traditional and cutting-edge techniques most commonly used in current life science research. Emphasis is placed on understanding the theory behind the techniques, as well as analysis of the resulting data. New chapters cover proteomics, genomics, metabolomics, bioinformatics, as well as data analysis and visualisation. Using accessible language to describe concepts and methods, and with a wealth of new in-text worked examples to challenge students' understanding, this textbook provides an essential guide to the key techniques used in current bioscience research.