This book contains a collection of papers on the molecular biology of the band 3 proteins and their various functions: as anion transporters, binding proteins for membrane skeleton, hemoglobin and glycolytic enzymes, and as a recognition signal for the removal of senescent cells by the immuno-system of the body. The papers presented were written to provide an overview of the work carried out during the past five years in most of the laboratories engaged in research on band 3. They serve to give the reader factual information on nearly all aspects of band 3 research, to introduce him to the current literature and to give him a feeling for the philosophy behind the approaches chosen in the various laboratories.

This second edition of Membrane Protein Purification and Crystallization, A Practical Guide is written for bench scientists working in the fields of biochemistry, biology, and proteomic research. This guide presents isolation and crystallization techniques in a concise form, emphasizing the critical aspects unique to membrane proteins. It explains the principles of the methods and provides protocols of general use, permitting researchers and students new to this area to adapt these techniques to their particular needs. This edition is not only an update but is comprised mainly of new contributions. It is the first monograph compiling the essential approaches for membrane protein crystallization, and emphasizes recent progress in production and purification of recombinant membrane proteins. Provides general guidelines and strategies for isolation and crystallization of membrane proteins Gives detailed protocols that have wide application, and low specialized equipment needs Emphasizes recent progress in production and purification of recombinant membrane proteins, especially of histidine-tagged and other affinity-epitope-tagged proteins Summarizes recent developments of Blue-Native PAGE, a high resolution separation technique, which is independent of the use of recombinant techniques, and is especially suited for proteomic analyses of membrane protein complexes Gives detailed protocols for membrane protein crystallization, and describes the production and use of antibody fragments for high resolution crystallization Presents a comprehensive guide to 2D-crystallization of membrane proteins

Biophysics is the science of physical principles underlying the "phenomenon of life" on all levels of organization. This book begins by explaining molecular and ionic interactions, movements, excitation and energy transfer, and the self-organization of supramolecular structures. Then the biological organism is introduced as a non-equilibrium system. Finally, system analyses are discussed as well as environmental biophysics, ecological interactions, growth, differentiation, and evolution. A growing number of applications in biotechnology are based on these biophysical concepts.

Nuclear magnetic resonance (NMR) is an analytical tool used by chemists and physicists to study the structure and dynamics of molecules. In recent years, no other technique has grown to such importance as NMR spectroscopy. It is used in all branches of science where precise structural determination is required and where the nature of interactions and reactions in solution is being studied. Annual Reports on NMR has established itself as a premier means for the specialist and nonspecialist alike to become familiar with new techniques and applications of NMR spectroscopy. Includes comprehensive review articles on NMR Spectroscopy NMR is used in all branches of science No other technique has grown to such importance as NMR Spectroscopy in recent years

It is easy to imagine the excitement that pervaded the neurological world in the late 1920's and early 1930's when Berger's first descriptions of the electro encephalogram appeared. Berger was not the first to discover that changes in electric potential can be recorded from the surface of the head, but it was he who first systematized the method, and it was he who first proposed that explanatory correlations might be found between the electroencephalogram, brain processes, and behavioral states. An explosion of activity quickly fol lowed: studies were made of the brain waves in virtually every conceivable behavioral state, ranging from normal human subjects to those with major psychoses or with epilepsy, to state changes such as the sleep-wakefulness transition. There evolved from this the discipline of Clinical Electroencepha lography which rapidly took a valued place in clinical neurology and neuro surgery. Moreover, use of the method in experimental animals led to a further understanding of such state changes as attention-inattention, arousal, and sleep and wakefulness. The evoked potential method, derived from electro encephalography, was used in neurophysiological research to construct pre cise maps of the projection of sensory systems upon the neocortex. These maps still form the initial guides to studies of the cortical mechanisms in sensation and perception. The use of the event-related potential paradigm has proved useful in studies of the brain mechanisms of some cognitive functions of the brain.